High-resolution DNA melting analysis: advancements and limitations
نویسندگان
چکیده
منابع مشابه
Comparison of cattle BoLA-DRB3 typing by PCR-RFLP, direct sequencing, and high-resolution DNA melting curve analysis
Major histocompatibility complex (MHC) represents an important genetic marker for manipulation to improve the health and productivity of cattle. It is closely associated with numerous disease susceptibilities and immune responses. Bovine MHC, also called bovine leukocyte antigen (BoLA), is considered as a suitable marker for genetic diversity studies. In cattle, most of the polymorphisms are lo...
متن کاملDNA concentration can specify DNA melting point in a high-resolution melting analysis master mix.
1. Algeciras-Schimnich A, Bruns DE, Boyd JC, Bryant SC, LaFortune KA, Grebe SKG. Failure of current laboratory protocols to detect lot-to-lot reagent differences: findings and possible solutions. Clin Chem 2013;59:1187–94. 2. Bais R. What information should manufacturers provide on their procedures? Clin Chem 2006; 52:1624–5. 3. Bais R, Armbruster D, Jansen RTP, Klee G, Panteghini M, Passarelli...
متن کاملبررسی حساسیت و اختصاصیت روش high resolution melting analysis در شناسایی انواع جهش های مختلف ژن rb1
روش های متنوعی برای شناسایی جهش ها ابداع شده است که هریک از آنها دارای مزایا و معایب خاص خود هستند. یکی از روش هایی که اخیراً ابداع شده و در مطالعات مختلف به کارآیی بالای آن اشاره شده است روش high resolution melting analysis یا hrm است. در مطالعه حاضر برای اولین بار در ایران به منظور شناسایی جهش ها تمامی اگزون های ژن rb1 با استفاده از روش تعیین توالی بررسی شده است. در ادامه به منظور بررسی حساسیت...
Detection of Microsatellite Instability by High-Resolution Melting Analysis in Colorectal Cancer
Background: Colorectal cancer (CRC) is the third most common cancer worldwide. microsatellite instability (MSI) is a molecular marker of a deficient mismatch repair system and happens in almost 15% of CRCs. Because of a wide frequency of MSI+ CRC in Iran compared to other parts of the world, the importance of screening for this type of cancer is highlighted. Methods: The most common MSI detecti...
متن کاملMicrobiological applications of high-resolution melting analysis.
High-resolution melting (HRM) analysis uses real-time PCR instrumentation to interrogate DNA sequence variation and is a low-cost, single-step, closed-tube method. Here we describe HRM technology and provide examples of varied clinical microbiological applications to highlight the strengths and limitations of HRM analysis.
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ژورنال
عنوان ژورنال: Human Mutation
سال: 2009
ISSN: 1059-7794,1098-1004
DOI: 10.1002/humu.20951